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I embroidered a 6" by 5.5" patch in the upper corner of my shirt, using four colors of embroidery thread, with the darkest threads used at the top, and the lightest ones used at the bottom. I used all 6 threads for each color, in order to ensure that my lines and stitches were thick enough to be visible. 

I chose to embroider this particular shirt, because it was fairly plain, and it didn't stretch as much as some of my other shirts, which would have made it much more difficult to embroider without first using interfacing.